Misha B. Ahrens, Jennifer M. Li, Michael B. Orger, Drew N. Robson, Alexander F. Schier, Florian Engert & Ruben Portugues
It is the first article of Whole-Brain Calcium Imaging.
- whole-brain calcium imaging
- elavl3:GCaMP2 Tg zebrafish
- motor adaptation
- larval zebrafish
- 3D recnstruction
- two photon microscopy (3Hz)
Sumarry
The size of larval zebrafish is enough to small to reach the bottom of the brain with 2phton microscopy ( > 1mm).
By using this animal, they achieved to whole-brain (all neuron in central nerve system) imaging of a behaving animal with single-cell resolution.
To achieve this, at first, they took time lapse images of calcium indicator (GCaMP2) at multiple regions using same behavior paradigm.
After that they reconstruct these time lapse images to reference brain.
They recorded calcium responses of larval zebrafish brain during swimming.
Using motor-visual feedback system, they mimicked a strong current (low gain) or weak current (high gain).
They found that
- States of neural activities change transiently triggered by change of motor visual feedback gain.
- Response neurons can be classified into 4types; motor-related, motor-off-related, gain-decreased-related, gain-increased-related.
- and also detected the position of these neurons in whole brain.
Comments
I feel that impact of this article is about technical issue, not scientific issue.
This work is big foot print for whole-brain imaging, one of our (my?) dream.
Of course, there are many problems and limitations.
For example, low sampling ratio, the number of sampling pictures, analysis methods and so on.
The biggest interest for me is "how to apply for mice (and other mammalians)".
To solve these problems, we should improve sensitivity for signal, S/N ratio, laser properties, laser scanning method, lens, image acquisition method, statistical methods for population analysis and ... one by one.
It remain (or increase) many chances to impact to this filed now!
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