Fast two-photon in vivo imaging with three-dimensional random-access scanning in large tissue volumes

Nature Methods | Article
Fast two-photon in vivo imaging with three-dimensional random-access scanning in large tissue volumes
Gergely Katona, Gergely Szalay, Pál Maák, Attila Kaszás, Máté Veress, Dániel Hillier, Balázs Chiovini, E Sylvester Vizi, Botond Roska & Balázs Rózsa

In this article, the Authors generated two photon microscopy using 2 pairs of AODs (audio-optical deflectors). The sampling ratio is over 20k Hz and recording area is 700(x) x 700(y) x 1400(z) um. It makes us to record the neural population activities with more large number of cells and high time resolution. Combing with other techniques, we may achieve recording single or a few columns level activity.

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